Key Features

Sensitive
Limit of detection down to 1% VAF

User Friendly
Matched normal not required
Included software reports MSI-H, MSI-L/MSS

Fast Turnaround
Results in less than two hours
Batch up to 46 samples per qPCR run

Versatile
Compatible with tumor tissue or cell-free DNA (cfDNA)
Microsatellite Instability Markers Covered
BAT-25, BAT-26, NR-21, NR-24, and MONO-27
Sample Data
- MSI Determination
- The MSI status is based on the Ct values for each marker in the sample, by comparing with the GAPDH internal control to normalize the sample input. If the Ct value of marker is earlier than threshold, the marker is a positive. And based on the number of positive markers out of the 5 markers, we can call the sample MSI-H (>2/5); MSI-L(1/5) or MSS(0/5). Figure is based on hypothetical data.
- MSI Software
- We provide an easy-to-use software to interpret MSI results and generate a report. Simply upload the qPCR data into the NuProbe MSI software, and the software gives sample results in a plate view map by color code. Users can then label the samples, view the result for each marker and qPCR curves, and export the MSI status report in Excel format.
Workflow

Step 1: DNA Extraction
DNA is extracted from clinical samples with commercial extraction kits such as cobas® cfDNA Sample Preparation Kit, QIAamp® DNA Blood Mini Kit, GeneRead® DNA FFPE Kit.

Step 2: qPCR BDA Reaction
NuProbe’s qPCR kit reagents and clinical samples are prepared for qPCR reactions. After running the reaction, Ct results are obtained.
1.5 hrs (15 min hands-on)

Step 3: Results Interpretation
The MSI status of each sample will be visualized and reported by the easy-to-use VarTrace MSI software.
2 min hands-on
Download Product User Manual
Compatible Instruments

BioRad CFX96
The assay has been optimized with BioRad CFX96. To use other qPCR instruments, please contact us.
Specifications

Catalog Number
1R-0401-S
48 Reactions
Technology | multi-blocker mBDA & qPCR |
Kit size | 48 specimens / kit |
Biomarkers | Tube 1: BAT26, NR21, NR24 |
Tube 2: BAT25, Mono27 | |
Internal Control | GAPDH |
Tubes/wells per specimen | 2 |
Turnaround time | qPCR only: 1h30min including approximately 15min hands-on time |
Limit of detection | ≥ 10ng per reaction: 1% |
< 10ng per reaction: 5% | |
Sensitivity | >98% |
Specificity | >98% at 1% VAF |
Specimen type | Blood or FFPE |
Specimen input | 1.5-15 ng per reaction (3-30 ng per specimen) ideally 20ng (10 ng per tube) |
Control | Positive (5%) and Negative |
Amplicon length | [80, 120] bp |
Storage condition | -20°C, up to 6 months |
Instruments | BioRad CFX96 |
Software | Included software reports MSS, MSI-L, MSI-H based on N/5 abnormal |
Request More Info
Alessandro Pinto, Ph.D., Director of Service and Partnership at NuProbe.
Fill out the form to contact Ale for questions about the VarTrace products.
